期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 23, 期 14, 页码 -出版社
MDPI
DOI: 10.3390/ijms23147717
关键词
GST; genome-wide; gene family; teinturier grape; anthocyanin
资金
- National Key RD Program [2020YFD100204]
- National Modern Agricultural Grape Industry Technology System [CARS-29]
- Jiangsu Agricultural Industrial Technology System [JATS [2021]450]
- Jiangsu Province Agricultural Major New Variety Creation Project [PZCZ201723]
- National Natural Science Foundation of China [31972384, 31901975]
This study analyzed and characterized the GST gene family in the flesh of 'Zhongshan-HongYu' grapes. The results showed that these genes were unevenly distributed on most chromosomes of the grapes and were divided into 9 subfamilies through phylogenetic analysis. Transcriptome sequencing analysis identified 18 GST genes, and their expression levels were found to be non-tissue-specific. The study also found a close relationship between VvGST4 and two other genes, providing a foundation for further research on the GST gene family.
Anthocyanins are synthesized in the endoplasmic reticulum and then transported to the vacuole in plants. Glutathione S-transferases (GSTs) are thought to play a key role in anthocyanin transport. To clarify the mechanism of GST genes in the accumulation and transport of anthocyanin in the early fruit stage, we analyzed and characterized the GST family in the flesh of 'Zhongshan-HongYu' (ZS-HY) based on the transcriptome. In this study, the 92 GST genes identified through a comprehensive bioinformatics analysis were unevenly present in all chromosomes of grapes, except chromosomes 3, 9 and 10. Through the analysis of the chromosomal location, gene structure, conserved domains, phylogenetic relationships and cis-acting elements of GST family genes, the phylogenetic tree divided the GST genes into 9 subfamilies. Eighteen GST genes were screened and identified from grape berries via a transcriptome sequencing analysis, of which 4 belonged to the phi subfamily and 14 to the tau subfamily, and the expression levels of these GST genes were not tissue-specific. The phylogenetic analysis indicated that VvGST4 was closely related to PhAN9 and AtTT19. This study provides a foundation for the analysis of the GST gene family and insight into the roles of GSTs in grape anthocyanin transport.
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