4.7 Article

Role of a ZF-HD Transcription Factor in miR157-Mediated Feed-Forward Regulatory Module That Determines Plant Architecture in Arabidopsis

期刊

出版社

MDPI
DOI: 10.3390/ijms23158665

关键词

ChIP-Seq; feed-forward loop (FFL); gene regulatory network (GRN); HB34; miRNA; next-generation sequencing; shoot branching

资金

  1. Plasma Advanced Technology for Agriculture and Food (Plasma Farming) through the Korea Institute of Fusion Energy (KFE) - Government funds, Republic of Korea [EN2225-9]
  2. National Research Foundation of Korea (NRF) - Korea government [NRF-2021R1A2C1005650]
  3. USDA ARS CRIS [190721000-030-00D]
  4. NSF [MCB-1900567]
  5. NIH [GM60396]
  6. National Research Council of Science & Technology (NST), Republic of Korea [EN2225] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

向作者/读者索取更多资源

Zinc finger homeodomain (ZF-HD) transcription factors have important roles in the growth, development, and architecture regulation of plants. In Arabidopsis, ZF-HD TFs negatively regulate miR157 and positively regulate SPL10, creating a feed-forward loop that constitutes a miRNA regulatory module.
In plants, vegetative and reproductive development are associated with agronomically important traits that contribute to grain yield and biomass. Zinc finger homeodomain (ZF-HD) transcription factors (TFs) constitute a relatively small gene family that has been studied in several model plants, including Arabidopsis thaliana L. and Oryza sativa L. The ZF-HD family members play important roles in plant growth and development, but their contribution to the regulation of plant architecture remains largely unknown due to their functional redundancy. To understand the gene regulatory network controlled by ZF-HD TFs, we analyzed multiple loss-of-function mutants of ZF-HD TFs in Arabidopsis that exhibited morphological abnormalities in branching and flowering architecture. We found that ZF-HD TFs, especially HB34, negatively regulate the expression of miR157 and positively regulate SQUAMOSA PROMOTER BINDING-LIKE 10 (SPL10), a target of miR157. Genome-wide chromatin immunoprecipitation sequencing (ChIP-Seq) analysis revealed that miR157D and SPL10 are direct targets of HB34, creating a feed-forward loop that constitutes a robust miRNA regulatory module. Network motif analysis contains overrepresented coherent type IV feedforward motifs in the amiR zf-HD and hbq mutant background. This finding indicates that miRNA-mediated ZF-HD feedforward modules modify branching and inflorescence architecture in Arabidopsis. Taken together, these findings reveal a guiding role of ZF-HD TFs in the regulatory network module and demonstrate its role in plant architecture in Arabidopsis.

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