Stropharia rugoso-annulata acetylated polysaccharides alleviate NAFLD via Nrf2/JNK1/AMPK signaling pathways

The acetylated Stropharia rugoso-annulata polysaccharides (ASRP) was successfully characterized, and the effects and mechanism on alleviating NAFLD were investigated in HFD-induced mice models. The characterization showed that ASRP was successfully acetylated and rich in galactose. The animal studies demonstrated that ASRP at the dose of 400 mg/kg possessed hepatoprotective effects by potential antioxidation, anti-inflammation and improving hepatocellular histopathology, with the possible mechanisms on regulating the JNK1/AP-1 and activating the Nrf2 signaling pathways. Besides, ASRP could improve the fat metabolism by activating the AMPK/SREBP-1c signaling pathways. The results provided basal theories for the development of ASRP on treating the NAFLD and its complications.

Automated summary

In this study, acetylated Stropharia rugoso-annulata polysaccharides (ASRP) were successfully characterized and found to have potential hepatoprotective effects by reducing oxidative stress, inflammation, and improving liver histopathology. Additionally, ASRP could also improve fat metabolism through the regulation of signaling pathways. These findings provide theoretical basis for the development of ASRP as a treatment for non-alcoholic fatty liver disease (NAFLD) and its complications.