期刊
INTERNATIONAL IMMUNOPHARMACOLOGY
卷 108, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.intimp.2022.108869
关键词
Lipopolysaccharide; Acute lung injury; Sevoflurane; AMP-activated protein kinase; Unc-51 like autophagy activating kinase 1; Protective autophagy
Sevoflurane suppresses apoptosis and inflammation by activating protective autophagy, thereby ameliorating LPS-induced ALI. The AMPK/ULK1/PIKFYVE pathway plays a key role in this process.
Background: Sevoflurane anesthesia is deemed as potential therapeutic drug for lipopolysaccharide (LPS)-induced acute lung injury (ALI), but the molecular mechanisms have not been fully delineated. Aim: The present study explored the specific molecular mechanism of sevoflurane regulating autophagy to reduce LPS induced ALI. Methods: Male C57BL/6J mice and mouse pulmonary microvascular endothelial cells (MPVECs) were treated with LPS to construct ALI models, and the levels of inflammation, apoptosis and autophagy were detected after treatment with sevoflurane. Meanwhile, cells were treated with autophagy inhibitor or AMP-activated protein kinase (AMPK)/unc-51 like autophagy activating kinase 1 (ULK1) pathway inhibitor in vitro to detect their effects on cell survival. Results: Sevoflurane reduced inflammation, recovered cell division so as to suppress cell apoptosis and maintain cell survival, and activated autophagic flux in LPS-induced ALI models in vivo and in vitro. Of note, the suppressing effects of sevoflurane on LPS-induced cell death were abrogated by inhibiting autophagy. Moreover, we evidenced that sevoflurane promoted activation of the AMPK/ULK1 pathway in LPS-induced ALI models. Blockage of this pathway abrogated the promoting effects of sevoflurane on cell autophagy and cell viability in LPS-treated cells. Conclusion: Collectively, sevoflurane suppresses apoptosis and inflammation via activating protective autophagy, thereby ameliorating LPS-induced ALI, and the AMPK/ULK1/ PIKFYVE pathway is responsible for the process.
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