期刊
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
卷 80, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.ifset.2022.103078
关键词
Photodynamic inactivation; Curcumin; Penicillium expansum; Mycelium; Fungistasis; Patulin biosynthesis
资金
- National Natural Science Foundation of China [32172275]
- Natural Science Foundation of Shandong Province [ZR2019BC049]
- China Postdoctoral Science Foundation [2018M642705]
This study found that curcumin-based photodynamic inactivation can effectively inhibit the growth of Penicillium expansum mycelium and the secretion of patulin, showing potential for application on fruits and vegetables, reducing resource waste.
Patulin (PAT) contamination caused by Penicillium expansum growth in postharvest fruit brings safety problems. This study investigated the inhibitory effect and mechanism of curcumin (CUR)-based photodynamic inactivation (PDI) on PAT secreted by P. expansum in vitro using a multifunctional light source instrument. After being treated with photosensitive CUR, the growth of mycelium was completely inhibited on potato dextrose agar medium, and the maximum PAT reduction of 93.06% can be achieved (330.0 J/cm(2), 300 mu M CUR) in liquid medium. The inherent mechanism was that PDI causes the reactive oxygen species accumulation in cells, which destroyed the antioxidant defense system and induced severe oxidative damage, resulting in the loss of cell integrity. Furthermore, mycelial apoptosis caused up-regulation of five genes, including PatB, PatE, PatG, PatI, and PatL, but down-regulated ten genes in the PAT biosynthetic pathway. These findings suggest that CUR-based PDI can inhibit fungal infection and toxin secretion. Industrial relevance: This study found that photosensitized curcumin can significantly inhibit mycelial growth and the secretion of patulin by Penicillium expansum. Curcumin-based photodynamic inactivation has the feasibility of the application on fresh fruits and vegetables, such as apples or oranges, and can avoided excessive waste of resources in picking, transporting, storing, and selling.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据