期刊
GENOME RESEARCH
卷 32, 期 8, 页码 1448-1462出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1101/gr.276395.121
关键词
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资金
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB31020000]
- National Key R&D Program of China (China's Ministry of Science and Technology [MoST]) grant [2018YFC 1406901]
- Chinese Academy of Sciences [152453KYSB20170002]
- Carlsberg Foundation [CF16-0663]
- Villum Foundation [25900]
- La Caixa Foundation [100010434, LCF/BQ/DE16/11570011]
- Instituto de Salud Carlos III (ISCIII) [PT17/0019]
- European Regional Development Fund (ERDF)
- Secretaria d'Universitats i Recerca del Departament d'Economia i Coneixement de la Generalitat de Catalunya [2017SGR595]
- European Research Council (ERC) under the European Union [864203]
- MINECO/FEDER, UE [BFU2017-86471-P]
- Agencia Estatal de Investigacion (AEI) [CEX2018000792-M]
- Howard Hughes International Early Career
- National Institutes of Health [1R01HG010898-01A1]
- Secretaria d'Universitats i Recerca and Centres de Recerca de Catalunya (CERCA) Programme del Departament d'Economia i Coneixement de la Generalitat de Catalunya [GRC 2017 SGR 880]
- European Research Council (ERC) [864203] Funding Source: European Research Council (ERC)
Transcriptomic diversity plays a significant role in disease, lineage-specific biology, and environmental adaptation. This study combines long- and short-read sequencing with mass spectrometry proteomics to analyze human, great apes, and rhesus macaque lymphoblastoid cell lines, resulting in the largest primate full-length isoform catalog to date. The findings reveal numerous transcriptomic innovations and isoform usage changes related to immune function and immunological disorders.
Transcriptomic diversity greatly contributes to the fundamentals of disease, lineage-specific biology, and environmental adaptation. However, much of the actual isoform repertoire contributing to shaping primate evolution remains unknown. Here, we combined deep long- and short-read sequencing complemented with mass spectrometry proteomics in a panel of lymphoblastoid cell lines (LCLs) from human, three other great apes, and rhesus macaque, producing the largest full-length isoform catalog in primates to date. Around half of the captured isoforms are not annotated in their reference genomes, significantly expanding the gene models in primates. Furthermore, our comparative analyses unveil hundreds of transcriptomic innovations and isoform usage changes related to immune function and immunological disorders. The confluence of these evolutionary innovations with signals of positive selection and their limited impact in the proteome points to changes in alternative splicing in genes involved in immune response as an important target of recent regulatory divergence in primates.
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