Identification of new stock [Matthiola incana (L.) R. Br] cultivars with high fertility through morphological and molecular markers

Garden stock [Matthiola incana (L.) R. Br.] is a commercially important horticultural crop owing to its ornamental effect. There are different stock cultivars varied in color and shape, especially flowered phenotype is an essential index evaluating its commercial value, because double flowered individuals have more brilliant flowers compared to single flowered one. The present work aimed: (1) to make superior cultivars with different colors, high fertility, being capable of early selecting only double flowered seedlings by leaf color and to investigate morphological characteristics and (2) to select RAPD and ISSR primers for the cultivar certification and identification to culture and produce good commercial stock cultivars. Here, in this study new stock cultivars were obtained with different colors including pink, pale pink and white, through outcrossing between white cultivar (high fertile but unable to select double flower phenotype) and pink cultivar (vice. versa). Among newly obtained stock cultivars, single and double flowered seedlings are distinguishable from single ones by leaf color, having about 70% of fertility. Moreover RAPD and ISSR markers selected in this study can be applied to identify different stock cultivars in seed production, culture and to establish cultivar certification system.

Automated summary

In this study, new garden stock cultivars with different colors were obtained through outcrossing, and high fertile double flowered seedlings were successfully selected by leaf color. The RAPD and ISSR markers selected can be applied for cultivar certification and identification, contributing to the culture and production of good commercial stock cultivars.