期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 187, 期 -, 页码 1-16出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2022.05.010
关键词
Polycystic ovary syndrome; Granulosa cells; Sirtuin 3; Mitochondria; Glucose metabolism; Oocyte quality
This study investigated the alterations in mitochondrial function and glucose metabolism in ovarian granulosa cells (GCs) of women with polycystic ovary syndrome (PCOS) and the role of SIRT3 in this process. The results showed that women with PCOS had impaired oocyte quality, accompanied by mitochondrial dysfunction, oxidative stress, and glucose metabolism defects in GCs. SIRT3 deficiency in GCs of PCOS patients may contribute to these abnormalities.
Mitochondrial function and glucose metabolism play important roles in bidirectional signaling between granulosa cells (GCs) and oocytes. However, the factors associated with mitochondrial function and glucose metabolism of GCs in polycystic ovary syndrome (PCOS) are poorly understood, and their potential downstream effects on oocyte quality are still unknown. The aim of this study was to investigate whether there are alterations in mitochondrial-related functions and glucose metabolism in ovarian GCs of women with PCOS and the role of Sirtuin 3 (SIRT3) in this process. Here, we demonstrated that women with PCOS undergoing in vitro fertilization and embryo transfer had significantly lower rates of metaphase II oocytes, two-pronuclear fertilization, cleavage, and day 3 good-quality embryos. Germinal vesicle-and metaphase I-stage oocytes from women with PCOS exhibited increased mitochondrial reactive oxygen species (ROS), decreased mitochondrial membrane potential, and downregulation of glucose-6-phosphate dehydrogenase. GCs from women with PCOS presented significant alterations in mitochondrial morphology, amount, and localization, decreased membrane potential, reduced adenosine triphosphate (ATP) synthesis, increased mitochondrial ROS and oxidative stress, and insufficient oxidative phosphorylation (OXPHOS) together with decreased glycolysis. SIRT3 expression was significantly decreased in GCs of PCOS patients, and knockdown of SIRT3 in KGN cells could mimic the alterations in mitochondrial functions and glucose metabolism in PCOS GCs. SIRT3 knockdown changed the acetylation status of NDUFS1, which might induce altered mitochondrial OXPHOS, the generation of mitochondrial ROS, and eventually defects in the cellular insulin signaling pathway. These findings suggest that SIRT3 deficiency in GCs of PCOS patients may contribute to mitochondrial dysfunction, elevated oxidative stress, and defects in glucose metabolism, which potentially induce impaired oocytes in PCOS.
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