期刊
FOOD RESEARCH INTERNATIONAL
卷 157, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.foodres.2022.111242
关键词
High-throughput sequencing; Food metagenome; Food microbiota; Food safety; Food spoilage; Omics
资金
- European Union [861915]
The diffusion of high-throughput sequencing has significantly impacted the study of food microbial ecology, allowing for a better understanding of microbial communities through amplicon-based and shotgun metagenomics approaches. Moreover, retrieving Metagenome-Assembled Genomes (MAGs) enables strain-tracking and response to food poisoning outbreaks.
The diffusion of high-throughput sequencing has dramatically changed the study of food microbial ecology. Amplicon-based description of the microbial community may be routinary implemented in the food industry to understand how the processing parameters and the raw material quality may affect the microbial community of the final product, as well as how the community changes during the shelf-life. In addition, application of shotgun metagenomics may represent an invaluable resource to understand the functional potential of the microbial community, identifying the presence of spoilage-associated activities or genes related to pathogenesis. Finally, retrieving Metagenome-Assembled Genomes (MAGs) of relevant species may be useful for strain-tracking along the food chain and in case of food poisoning outbreaks. This review gives an overview of the possible applications of sequencing-based approaches in the study of food microbial ecology, highlighting limitations that still prevent the spreading of these techniques to the food industry.
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