期刊
FOOD CONTROL
卷 137, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2022.108971
关键词
S; Typhimurium; L; monocytogenes; Real-time PCR; Adhesion; dry conditions; Surfaces
资金
- Ministerio de Ciencia, Innovacion y Universidades [RTI2018-098267-R-C32]
This study evaluated the capacity of real-time PCR and conventional culture methods to detect Salmonella enterica serovar Typhimurium and Listeria monocytogenes adhered to stainless steel surfaces used as food contact surfaces. Real-time PCR showed a higher detection capacity for low concentration samples, and even detected samples without pre-enrichment. However, pre-enrichment is still recommended to avoid false positive and false negative results.
This study evaluated the capacity of real-time PCR and conventional culture methods to detect Salmonella enterica serovar Typhimurium and Listeria monocytogenes adhered to stainless steel surfaces used as food contact surfaces. The adhesion of the microorganisms to the surfaces was performed under dry conditions to represent the stress to which these pathogens can be subjected in the food processing environment. The samples were analyzed with various pre-enrichment times: S. Typhymurium (0, 6, and 18 h) and L. monocytogenes (0, 6, and 25 h) and with procedures concentrating or not concentrating the samples after the pre-enrichments. The results showed that real-time PCR obtained increased capacity than the conventional method to detect a low number of both pathogens, and real-time PCR even detected samples without pre-enrichment. However, pre-enrichment is recommended to avoid the detection of false positives from dead cells during adhesion and to ensure the absence of false negatives due to low initial concentrations. The concentration of the adhering bacteria increased the frequency in the detection of positive results for S. Typhimurium, but this effect was not observed in the case of L. monocytogenes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据