Effects of protein digestion on in vitro digestibility of starch in sorghum differing in endosperm hardness and flour particle size

In vitro digestibility of starch in sorghum grains differing in endosperm hardness and flour particle size was investigated. The starch digestibility increased as the particle size of flour decreased, but no clear trend was observed in digestibility of starch in sorghum flours milled from grains with different hardness. The protein matrix affected the digestion of starch. The pH value (2.0 vs. 1.3) was a critical factor affecting protein digestion. Optimum pH (pH 2.0 for pepsin) digested more protein, resulting in a greater digestion of starch. Resistant starch (RS) content was 8.5-26.3% in isolated sorghum starch but higher (10.6-29.5%) in sorghum flours. Protein digestibility decreased after cooking while starch digestibility increased compared to native sorghum flours; disulfide bonds formed between protein molecules. RS content of cooked sorghum flour was much higher without pepsin treatment (16.93-23.99%) than that of cooked sorghum flour with pepsin treatment (4.86-12.53%).

Automated summary

The digestibility of starch in sorghum grains was influenced by the particle size of flour and the hardness of the grains. The protein matrix and pH value also played important roles in starch digestion. The content of resistant starch varied in different forms of sorghum, with higher levels observed in sorghum flours. Cooking had different effects on protein and starch digestibility in sorghum flours.