In vivo absorptomics: Identification of bovine milk-derived peptides in human plasma after milk intake

A dedicated two-step purification procedure prior to nanoliquid chromatography-electrospray-tandem mass spectrometry analysis enabled the identification of bovine milk-derived peptides absorbed and circulating in the plasma of three healthy volunteers who received 250 mL of pasteurized milk after a 10-days washout. The appearance and clearance of milk peptides in plasma were monitored at various time points. Overall, 758, 273 and 212 unique peptides derived from 15, 15 and 18 bovine milk proteins, respectively, were identified in the plasma of these volunteers, evidencing a substantial inter-individual variability. Peptides encrypting possible bioactive and/or immunogenic molecules originating from caseins, beta-lactoglobulin and minor milk proteins were detected. Peptide representation data revealed the combined action of endoproteases involved in primary hydrolysis during gastroduodenal digestion and exopeptidases that hydrolyse peptides in the small intestine. It remains to be established whether the half-life and concentration ranges of circulating milk-derived peptides may have any impacts on human health.

Automated summary

A purification procedure followed by mass spectrometry analysis was used to identify milk-derived peptides in the plasma of healthy volunteers after consuming pasteurized milk. The study revealed individual variability in the presence of peptides from various bovine milk proteins, including caseins and beta-lactoglobulin. The potential bioactivity and immunogenicity of these peptides and their effects on human health require further investigation.