期刊
FOOD CHEMISTRY
卷 383, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2022.132404
关键词
Temporal sensing; Anodic dissolution; Cathodic biocatalysis; O-2 reduction reaction; S. aureus
资金
- National Natural Science Foundation of China [21804071]
- Natural Science Foundation of Jiangsu Province of China [BK20180688]
An Ag@C hybrid bipolar electrode sensing platform has been established for the detection of Staphylococcus aureus in food. This platform combines the advantages of anodic dissolution of Ag and cathodic biocatalysis of O-2 reduction, achieving an ultralow detection limit and quantification of S. aureus.
An Ag@C hybrid bipolar electrode (BPE) sensing platform has been established for the temporal detection of Staphylococcus aureus (S. aureus) in food. Combining the advantages of anodic dissolution of Ag and cathodic biocatalysis of oxygen (O-2) reduction, this strategy showed an ultralow detection limit down to 10 CFU mL(-1). As the formation of Ag@C completely quenched the electrochemiluminescence (ECL) emission of luminol, the ECL emission recovery reflected the extent of anodic dissolution. Meanwhile, S. aureus catalyzed the electrochemical reduction of O-2 at the cathode, reducing the overpotential for cathodic O-2 reduction and thus increasing the rate of anodic electron loss, facilitating Ag dissolution and restoring the ECL emission of luminol. When a constant potential was applied, through monitoring the ECL recovery time before and after the incubation of S. aureus on the cathode, S. aureus could be quantified due to the slight difference of the conductivity.
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