Simultaneous Voltammetric Determination of Tryptamine and Histamine in Wines Using a Carbon Paste Electrode Modified with Nickel Phthalocyanine

The biogenic amines, especially tryptamine (Tryp) and histamine (Hist), are toxicologically precarious for human health, causing intoxication. The combination of Tryp and Hist together presents even greater damage to human health, since Tryp increases the toxicity of Hist. Information about the presence of this amine is necessary to assess the quality of the wine and avoid intoxication caused by its ingestion. In this work, the electrocatalytic oxidation of Tryp and Hist on a carbon paste electrode modified with nickel phthalocyanine (CPE-NiPC) was studied, and a square wave voltammetric method was developed for simultaneous determination of these amines in wines. The use of NiPC contributed significantly to the peak current gain in the analytical response of the amines. The optimal response and sensitivity of the two analytes were obtained using phosphate buffer (pH 8.5), yielding a linear response range of 0.49-3.93 ng mL(-1) and 0.37-2.9 mu g mL(-1), a limit of detection of 0.168 ng mL(-1) and 0.038 mu g mL(-1) for Tryp and Hist, respectively. The proposed sensor CPE-NiPC has high selectivity affording greater accuracy in determining those amines and providing recovery values in a range of 82.65-103.02% for the analyzed biogenic amines.

Automated summary

In this study, a square wave voltammetric method was developed for simultaneous determination of tryptamine and histamine in wine using a carbon paste electrode modified with nickel phthalocyanine. The proposed sensor showed high selectivity and accuracy, providing recovery values within a range of 82.65-103.02% for the analyzed biogenic amines.