期刊
FISH & SHELLFISH IMMUNOLOGY
卷 131, 期 -, 页码 264-273出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.08.007
关键词
Hypoxia; Angiogenesis; Vascular endothelial growth factor; Hypoxia inducible factor; Micropterus salmoides
资金
- National Natural Science Foundation of China [31802267]
- Double Support Project fund of Sichuan Agricultural University (SICAU) [1921993232, 1921993229]
This study investigated the effect of acute hypoxia on liver angiogenesis in largemouth bass. The results showed that hypoxia exposure promoted angiogenesis occurrence and increased the concentration of vasodilation factors. Hypoxia exposure also up-regulated the expression of certain genes and proteins related to angiogenesis. The findings suggest that acute hypoxia can stimulate liver angiogenesis in largemouth bass through the HIF-dependent pathway.
A 24-h hypoxia exposure experiment was conducted to determine how hypoxia exposure induce liver angiogenesis in largemouth bass. Nitrogen (N-2) was pumped into water to exclude dissolved oxygen into 1.2 +/- 0.2 mg/L, and liver tissues were sampled during hypoxia exposure of 0 h, 4 h, 8 h, 12 h, 24 h and re-oxygenation for 12 h. Firstly, the results showed that hypoxia exposure promoted the angiogenesis occurrence by immunohistochemical analysis of vascular endothelial growth factor receptor 2 (VEGFR2). Secondly, the concentration of vasodilation factor increased and it's activity was elevated during 8 h exposure, such as nitric oxide (NO) and nitric oxide synthase (NOS) (p < 0.05). Thirdly, hypoxia exposure promoted angiogenesis through up-regulation the expression of matrix metalloproteinase 2 (MMP-2), jagged, protein kinase B (AKT), phosphoinositide-3-kinase (PI3K), mitogen-activated protein kinase (MAPK) at 4 h; contrarily, the expression of inhibiting angiogenesis genes presented up-regulated at 8 h (p < 0.05), such as matrix metalloproteinase inhibitor-2 (TIMP-2), matrix metalloproteinase inhibitor-3 (TIMP-3). Finally, the genes and proteins that regulate angiogenesis presented obvious chronological order. Parts of them promoted the budding and extension of blood vessels were upregulated during 4 h-8 h (p < 0.05), such as vascular endothelial growth factor a (VEGFA), VEGFR2, monocarboxylic acid transporter 1 (MCT1), CD147, prolyl hydroxylase (PHD), nuclear factor kappa-B (NF-kappa B); other part of them promoted blood vessel maturation were highly expressed during 12 h-24 h (p < 0.05), such as angiogenin-1 (Ang-1) and angiogenin-2 (Ang-2). In short, acute hypoxia can promote the liver angiogenesis of largemouth bass by HIF - dependent pathway.
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