期刊
FISH & SHELLFISH IMMUNOLOGY
卷 127, 期 -, 页码 375-385出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.06.049
关键词
Streptomyces; Aeromonas hydrophila; Aquaculture; Probiotics; Zebrafish
资金
- National Natural Science Foundation of China (NSFC) [42030713]
- Guangdong Basic and Applied Basic Research Foundation [2019A1515011791, 2019A1515012112, 2022A1515012499]
- Key Program of Marine Economy Development (Six Marine Industries) Special Foundation of Department of Natural Resources of Guangdong Province [GDNRC [2021] 37]
- Science and Technology Planning Project of Guangdong Province [[2019] 170, KTP20210324]
- Guangzhou Science and Technology Project [GZKTP202032]
- Guangzhou Basic and Applied Basic Research Foundation [202102080372]
Pre-treatment of Streptomyces sp. SH5 on zebrafish significantly enhanced larvae resistance against Aeromonas hydrophila, by inhibiting pathogen colonization, increasing microbial diversity, and strengthening immune response. However, it also decreased the expression of inflammatory stimulus genes.
Pre-treatment of Streptomyces sp. SH5 on zebrafish lead to a significant enhancement of larvae survival upon Aeromonas hydrophila challenging. SH5 was able to colonize in zebrafish approximately at 1 x 10(2.6) cells per fish for at least seven days. The presence of SH5 strongly repelled the A. hydrophila colonization in zebrafish, and maximally, a 67.53% reduction rate was achieved. A more diversified flora was discovered in the SH5-treated zebrafish larvae at both phylum and genus levels. The expression of immune response genes of SH5-treated zebrafish, including TLR3, lysozyme and NOS2 alpha, were enhanced at initial stage, while, that of various inflammatory stimuli genes including 1L-1 beta, 1L-6 and MyD88 were decreased at all tested timepoints. SH5 was shown to inhibit virulence factors production and the expression of corresponding virulence genes in A. hydrophila, suggesting its quorum sensing inhibitory potential. These results indicated favorable application perspectives of SH5 in resisting pathogenic infection in aquaculture.
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