期刊
FISH & SHELLFISH IMMUNOLOGY
卷 127, 期 -, 页码 982-990出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2022.07.026
关键词
Bacterial infection; Neutrophil; Neutrophils extracellular traps; Pyroptosis; Turbot
资金
- National Natural Science Foundation of China [32102836]
- Natural Science Foundation of Shanghai [20ZR1415500]
- Fundamental Research Funds for the Central Universities [222201221733]
This study reveals the important role of pyroptosis in the formation of NETs in turbot neutrophils, suggesting that NETs formation is a critical immune response during bacterial infection in teleost fish.
Neutrophils can capture and kill pathogens by releasing neutrophils extracellular traps (NETs), which play critical roles in anti-microbial infection in mammals; however, the mechanisms involved in NETs formation and its role in anti-bacterial infection in teleost fish remains largely unknown. In this study, to explore the function of NETs in turbot, we established an in vitro bacterial infection model in head kidney derived neutrophils, and found that the haemolysin over-expressed Edwardsiella piscicida (ethA(+)) could induce a robust phenotype of NETs, compared with that in wild type or ethA mutant (ethA(+) -Delta ethA) strains. Besides, the NETosis was mediated by ethA(+) -induced pyroptosis, and arms the ability of bacterial killing in neutrophils of turbot. Moreover, we found that neutrophils elastase (NE) might involves in this pyroptotic signaling, rather than inflammatory Smcaspase. Taken together, this study reveals the important role of pyroptosis in NETs formation in turbot neutrophils, suggesting that NETs formation is a critical immune response during bacterial infection in teleost fish.
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