期刊
FEBS LETTERS
卷 596, 期 19, 页码 2576-2590出版社
WILEY
DOI: 10.1002/1873-3468.14431
关键词
abscisic acid; biosensor; cell-based assay; synthetic biology
资金
- VIB International PhD Program
- Fund for Scientific Research-Flanders (FWO) [1SE0521N]
- FWO grant [G021119N]
- Ghent University Concerted actions (GOA) grant [BOF19GOA-004]
Abscisic acid (ABA) is a signaling molecule found in various organisms. Its pharmacological activities and potential as a biomarker for certain diseases have sparked interest in developing a method to quantify ABA levels in mammalian cells and tissues. Researchers have optimized a biosensor system based on the interaction between plant ABA receptors and co-receptors to detect low concentrations of ABA in HEK293T cells.
Abscisic acid (ABA) is a signalling molecule conserved in plants, bacteria, fungi, and animals. Recently, ABA has gained attention for its pharmacological activities and its potential as a biomarker for the severity of chronic obstructive pulmonary disease and glioma. This prompts the development of a reliable, sensitive, rapid, and cost-effective method to quantify ABA levels in mammalian cells and tissues. The previously described ABA biosensor system based on the ABA-dependent interaction between the plant ABA receptor PYL1 and co-receptor ABI1 is not sensitive enough for the low ABA levels seen in mammals. Therefore, we optimized this system by replacing PYL1 with other high-affinity plant PYL proteins. The optimized biosensor system engineered with the PYL8 receptor enabled the quantification of ABA at low concentrations in HEK293T cells.
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