期刊
FASEB JOURNAL
卷 36, 期 9, 页码 -出版社
WILEY
DOI: 10.1096/fj.202200332R
关键词
bile acid; hepatitis B virus; SOCE; T cell activation
资金
- China Postdoctoral Science Foundation [2021M693514]
- Chinese Academy of Medical Sciences (CAMS) [2021-I2M-5-011]
- National Key Research and Development Program of China [2021YFA1301300]
- National Natural Science Foundation of China (NSFC) [81720108032, 81930109, 81973559, 82173886]
- Overseas Expertise Introduction Project For Discipline Innovation [G20582017001]
- Sanming Project of Medicine in Shenzhen [SZSM201801060]
Cholestasis, a common complication of hepatitis B virus infection, has been found to negatively impact T cells response and hinder viral clearance. Bile acids disrupt calcium homeostasis, impairing T cells activation and signaling. Targeting bile acid regulation may be a therapeutic strategy for host-virus defense.
Cholestasis is a common complication of hepatitis B virus (HBV) infection, characterized by increased intrahepatic and plasma bile acid levels. Cholestasis was found negatively associated with hepatitis outcome, however, the exact mechanism by which cholestasis impacts anti-viral immunity and impedes HBV clearance remains elusive. Here, we found that cholestatic mice are featured with dysfunctional T cells response, as indicated by decreased sub-population of CD25(+)/CD69(+) CD4(+) and CD8(+) cells, while CTLA-4(+) CD4(+) and CD8(+) subsets were increased. Mechanistically, bile acids disrupt intracellular calcium homeostasis via inhibiting mitochondria calcium uptake and elevating cytoplasmic Ca2+ concentration, leading to STIM1 and ORAI1 decoupling and impaired store-operated Ca2+ entry which is essential for NFAT signaling and T cells activation. Moreover, in a transgenic mouse model of HBV infection, we confirmed that cholestasis compromised both CD4(+) and CD8(+) T cells activation resulting in poor viral clearance. Collectively, our results suggest that bile acids play pivotal roles in anti-HBV infection via controlling T cells activation and metabolism and that targeting the regulation of bile acids may be a therapeutic strategy for host-virus defense.
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