Periodontal ligament fibroblasts-derived exosomes induced by PGE(2) inhibit human periodontal ligament stem cells osteogenic differentiation via activating miR-34c-5p/SATB2/ERK

Several studies have confirmed that exosomes containing microRNAs (miRNAs) from the aseptic inflammatory microenvironment play an important role in bone remodeling. But the mechanism that induces changes in the osteogenic ability of periodontal ligament stem cells (PDLSCs) is still unclear. In the present study, the osteogenic function of periodontal ligament fibroblasts-derived exosomes induced by PGE(2) on PDLSCs was detected by real-time PCR, alizarin red assay and alkaline phosphatase staining. High-throughput miRNAs sequencing was used to reveal that miR-34c-5p in exosomes-PGE(2) was upregulated compared it in exosomes-normal. Real-time PCR and western blotting assay verified that overexpression of miR-34c-5p inhibited osteogenic differentiation, and reduced phosphorylation of ERK1/2. In addition, dual-luciferase reporter assay revealed that miR-34c-5p targeted special AT-rich sequence-binding protein 2 (SATB2). It was shown that exosomal miR-34c-5p inhibited osteogenic differentiation of PDLSCs via SATB2/ERK pathway.

Automated summary

Studies have shown that exosomes containing miRNAs from the aseptic inflammatory microenvironment play a crucial role in bone remodeling, but the mechanism affecting the osteogenic ability of PDLSCs remains unclear. This study demonstrated that exosomes derived from periodontal ligament fibroblasts, induced by PGE(2), inhibit osteogenic differentiation of PDLSCs through the miR-34c-5p/SATB2/ERK pathway.