期刊
EXPERIMENTAL BIOLOGY AND MEDICINE
卷 247, 期 15, 页码 1301-1316出版社
SAGE PUBLICATIONS LTD
DOI: 10.1177/15353702221098060
关键词
Aflatoxin B-1; Sorghum bicolor; male reproductive toxicity; hypothalamic-pituitary-gonadal axis; oxidative-inflammatory biomarkers; antioxidant activity
The study found that the API-enriched extract from Sorghum bicolor sheaths has a protective effect against AFB(1)-induced dysfunction of male rat's reproductive system. It improves hormone levels, antioxidant defenses, and reduces inflammation and apoptosis, ultimately leading to improved sperm quality and quantity.
We examined the protective effect of the apigeninidin (API)-enriched fraction from Sorghum bicolor sheaths extracts (SBE-05, SBE-06, and SBE-07) against aflatoxin B-1 (AFB(1))-induced dysregulation of male rat's reproductive system that may trigger infertility. Male rats (160 +/- 12 g) were treated with AFB(1) (50 mu g/kg) along with 5 or 10 mg/kg of SBE-05, SBE-06, and SBE-07 for 28 days. Subsequently, we assessed the reproductive hormone-prolactin, FSH, LH, testosterone levels, and testicular function enzymes. Moreover, we examined rats' testes, epididymis, and hypothalamus for oxidative and inflammatory stress biomarkers, caspase-9 activity and tissues pathology. We observed that comparative to AFB(1) alone treated rats, API co-treatment significantly (p < 0.05) abated the AFB(1)-mediated decrease in prolactin and antioxidant defenses and lessened lipid peroxidation (LPO) and reactive oxygen and nitrogen species levels in the examined organs-testes, epididymis, and hypothalamus. API abated AFB(1)-induced hormone decreases-testosterone, FSH, and LH; and caused improvement in sperm quantity and quality. API lessened AFB(1)-mediated increase in pro-inflammatory cytokine, increased interleukin-10 level, an anti-inflammatory cytokine and reduced caspase-9 activities. In addition, API reduced alterations in the examined tissue histology. Our findings suggest that S. bicolor API-enrich extracts have active antioxidative, antiapoptotic, and anti-inflammatory activities, which can protect against AFB(1)-induced dysfunction of the hypothalamic-pituitary-gonadal axis.
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