期刊
JOURNAL OF COMPARATIVE NEUROLOGY
卷 524, 期 17, 页码 3485-3502出版社
WILEY
DOI: 10.1002/cne.24078
关键词
cytoskeleton; neural stem cell; topography; laminin; microtubules; actin; migration; RRID: AB_561007; RRID: AB_531887; RRID: AB_305808; RRID: AB_2313773; RRID: AB_477010; RRID: AB_628431; RRID: SCR_014242; RRID: SCR_013672; RRID: SCR_002285; RRID: SCR_001905; RRID: SciRes_000136
资金
- National Center for Advancing Translational Sciences [8UL1TR000090-05]
- Pelotonia Foundation Undergraduate Research Fellowship
- Ohio State University/Metro High School Graduate Teaching Assistant Fellowship
We sought to determine the contribution of scaffold topography to the migration and morphology of neural stem cells by mimicking anatomical features of scaffolds found in vivo. We mimicked two types of central nervous system scaffolds encountered by neural stem cells during development in vitro by constructing different diameter electrospun polycaprolactone (PCL) fiber mats, a substrate that we have shown to be topographically similar to brain scaffolds. We compared the effects of large fibers (made to mimic blood vessel topography) with those of small-diameter fibers (made to mimic radial glial process topography) on the migration and differentiation of neural stem cells. Neural stem cells showed differential migratory and morphological reactions with laminin in different topographical contexts. We demonstrate, for the first time, that neural stem cell biological responses to laminin are dependent on topographical context. Large-fiber topography without laminin prevented cell migration, which was partially reversed by treatment with rock inhibitor. Cell morphology complexity assayed by fractal dimension was inhibited in nocodazole- and cytochalasin-D-treated neural precursor cells in large-fiber topography, but was not changed in small-fiber topography with these inhibitors. These data indicate that cell morphology has different requirements on cytoskeletal proteins dependent on the topographical environment encountered by the cell. We propose that the physical structure of distinct scaffolds induces unique signaling cascades that regulate migration and morphology in embryonic neural precursor cells. (C) 2016 Wiley Periodicals, Inc.
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