期刊
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
卷 237, 期 -, 页码 -出版社
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.ejmech.2022.114399
关键词
pyrimido[5,4-g]indolizines; pyrimido[4,5-c]pyrrolo[1,2-a]azepines; Triple negative human breast cancer; MDA-MB-231; Photosensitizing agents; Phototoxic activity
资金
- Ministero dell'Istruzione dell'Universita e della Ricerca (MIUR)
This study developed a class of new small molecule photosensitizing agents that showed strong anti-proliferative activity and high selectivity against triple negative and HER2 positive breast cancer cell lines. The compounds were able to increase reactive oxygen species production and induce apoptosis, making them promising candidates for phototherapy against aggressive and resistant cancers.
Searching for new small molecules as photosensitizing agents, we have developed a class of twenty-five pyrimido [5,4-g]indolizine and pyrimido[4,5-c]pyrrolo[1,2-alpha]azepines with a good substitution pattern defining a versatile synthetic pathway to approach the title ring system. All compounds were evaluated for their photocytotoxicity on a triple negative human breast cancer cell line (MDA-MB-231) in the dark and under UVA light (2.0 J/cm(2)). The most effective compounds exhibited a photoantiproliferative activity with IC50 values up to nanomolar ranges. Interestingly, these new developed compounds showed high selectivity towards cancerous cells with respect to non-cancerous ones. Moreover, four representative derivatives demonstrated to be phototoxic also against an additional human HER2 positive breast cancer cell line (HCC1954), and against the HER2 positive vesical cancer cell line (T24) harboring Hras mutation. Mechanistic studies performed in triple negative MDA-MB-231 cancer cells revealed the ability of the compounds to increase reactive oxygen species (ROS) production and to induce a thiol redox stress, thus triggering cancer cell death through apoptosis. Apoptotic cell death was also induced in highly aggressive and metastatic HER2 positive Hras mutated T24-treated bladder cancer cells. Overall, our data confirm that these new small photosensitizing agents may represent very promising candidates for phototherapy application against highly aggressive and resistant cancers.
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