期刊
EUROPEAN JOURNAL OF CELL BIOLOGY
卷 101, 期 3, 页码 -出版社
ELSEVIER GMBH
DOI: 10.1016/j.ejcb.2022.151227
关键词
Extracellular vesicles; Exosomes; Purification; Isolation methods; Nanomedicine; Theranostics; GMP manufacturing
类别
资金
- SGR programme of Generalitat de Catalunya [2017-SGR-301, 2017-SGR-483]
- Spanish Ministry of Economy and Competitiveness-MICINN [PID2019-110137RB-I00]
- ISCIII-REDinREN [RD16/0009]
- Red Traslacional para la Aplicacion Clinica de Vesculas Extracelulares [RED2018-102411-T]
- Red de Terapia Celular-TerCel [RD16/0011/0006]
- CIBER Cardiovascular [CB16/11/00403]
- Instituto Carlos III as part of the Plan Nacional de I+D+I [ICI20/00135, PI20/00097]
- Fondo Europeo de Desarrollo Regional (FEDER)
- Institut Catala de Salut (Generalitat de Catalunya)
- ISCIII [FI20/00021, MS19/00018]
- ERDF/ESF, Investing in your future
- Health Department of the Catalan Government (Generalitat de Catalunya)
Extracellular vesicles (EVs) have great potential in clinical application for disease research and biomarker development. However, standardized techniques for EV isolation and detailed reporting of methodology are crucial for ensuring reliable and comparable results.
Extracellular vesicles (EVs) are becoming promising tools for clinical application, either as sources of disease-specific molecular signatures for the unraveling of disease pathophysiology and establishment of novel bio-markers, or as platforms for cell-free nanotherapy. Yet, an unsolved issue is to define standardized techniques for EV isolation allowing data comparison across laboratories worldwide. Considering the difficulties to find this necessary consensus, it has to be stressed out that the outcome of the downstream analysis might be deeply biased by the isolation method, among other variables. Thus, it is crucial that the researcher is aware of the strengths and weaknesses of each method keeping their intended use in mind, and to sufficiently report the methodology details for the results to be comparable and solid. This review aims to present the most widely used EV isolation methods, from the initial differential ultracentrifugation (dUC) to newest approaches.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据