Effect of microbial agents on maturity, humification, and stability and the bacterial succession of spent mushroom substrate composting

Two composting experiments were conducted to investigate the effects of commercial microbial agents on microbial succession and nutrient flow such as humification, maturation, and stability during the aerobic composting of the spent mushroom substrate (SMS). The cellulose degradation rate of T (added microbial agents at the initial stage) reached 41.8%, which was much significantly (p < 0.05) higher than that of CK (14.9%). The seed germination index (GI) in T (82.38%) was significantly (p < 0.05) higher than that in CK (74.74%) in the maturation phase. Moreover, the total organic carbon/total nitrogen ratio (C/N) and electrical conductivity (EC) value of T decreased to 10.5 and 2.37 mS/cm, respectively. Chemical detection and fluorescence excitation-emission region integration method (EEM-FRI) analysis showed that the microbial agents significantly accelerated the organic matter (OM) decomposition and promoted the quality of mature compost using SMS as a single raw material. The bacterial abundance of T was significantly richer than the CK due to the addition of microbial agents. The results could provide a comprehensive understanding of adding microbial agents into composting SMS and a scientific feasibility strategy to rational utilization of resources in the edible fungi industry, which was conducive to the waste management and sustainable development of the edible fungi industry.

Automated summary

The study revealed that adding commercial microbial agents can significantly increase the cellulose degradation rate and seed germination index in spent mushroom substrate composting, promote organic matter decomposition, and enhance the quality of mature compost.