期刊
ENVIRONMENTAL SCIENCE & TECHNOLOGY
卷 56, 期 12, 页码 8197-8208出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.2c01602
关键词
non-steroidal anti-inflammatory drugs; chiral inversion; 2-arylpropionic acids; anaerobic waste treatment; microbial community; 2-arylpropionyl-CoA epimerase
资金
- Australian Research Council (ARC) [DP170100357]
- University of Technology Sydney
This study investigated the chiral inversion of 2-arylpropionic acids (2-APAs) and the associated microbial community under anaerobic conditions. The results showed diverse chiral inversion behaviors of 2-APAs, with some compounds exhibiting unidirectional inversion and others showing bidirectional inversion. Several bacterial genera were identified in the anaerobic digesters and predicted to have the gene involved in chiral inversion, but they were present at low abundances, explaining the limited extent of chiral inversion observed in this study.
This work examined the chiral inversion of 2-arylpropionic acids (2-APAs) under anaerobic conditions and the associated microbial community. The anaerobic condition was simulated by two identical anaerobic digesters. Each digester was fed with the substrate containing 11 either pure (R)- or pure (S)-2-APA enantiomers. Chiral inversion was evidenced by the concentration increase of the other enantiomer in the digestate and the changes in the enantiomeric fraction between the two enantiomers. Both digesters showed similar and poor removal of 2-APAs (<= 30%, except for naproxen) and diverse chiral inversion behaviors under anaerobic conditions. Four compounds exhibited (S -> R) unidirectional inversion [flurbiprofen, ketoprofen, naproxen, and 2-(4-tert-butylphenyl)propionic acid], and the remaining seven compounds showed bidirectional inversion. Several aerobic and facultative anaerobic bacterial genera (Candidatus Microthrix, Rhodococcus, Mycobacterium, Gordonia, and Sphingobium) were identified in both digesters and predicted to harbor the 2-arylpropionyl-CoA epimerase (enzyme involved in chiral inversion) encoding gene. These genera presented at low abundances, <0.5% in the digester dosed with (R)-2-APAs and <0.2% in the digester dosed with (S)-2-APAs. The low abundances of these genera explain the limited extent of chiral inversion observed in this study.
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