Effect of sulfur sources on the competition between denitrification and DNRA

The fate of nitrogen is controlled by the competition between nitrate reduction pathways. Denitrification removes nitrogen in the system to the atmosphere, whereas dissimilatory nitrate reduction to ammonia (DNRA) retains nitrate in the form of ammonia. Different microbes specialize in the oxidation of different electron donors, thus electron donors might influence the outcomes of the competition. Here, we investigated the fate of nitrate with five forms of sulfur as electron donors. Chemoautotrophic nitrate reduction did not continue after the passages of the enrichments with sulfide, sulfite and pyrite. Nitrate reduction rate was the highest in the enrichment with thiosulfate. Denitrification was stimulated and no DNRA was observed with thiosulfate, while both denitrification and DNRA were stimulated with elemental sulfur. Metagenomes of the enrichments were assembled and binned into ten genomes. The enriched populations with thiosulfate included Thiobacillus, Len-timicrobium, Sulfurovum and Hydrogenophaga, all of which contained genes involved in sulfur oxidation. Elemental sulfur-based DNRA was performed by Thiobacillus (with NrfA and NirB) and Nocardioides (with only NirB). Our study established a link between sulfur sources, nitrate reduction pathways and microbial populations.

Automated summary

The fate of nitrogen is influenced by competition between nitrate reduction pathways, with denitrification removing nitrogen to the atmosphere and DNRA retaining nitrate as ammonia. This study explored the impact of five sulfur forms as electron donors on nitrate fate. The results showed that thiosulfate had the highest nitrate reduction rate, stimulating denitrification and inhibiting DNRA. Elemental sulfur, on the other hand, stimulated both denitrification and DNRA. Metagenomic analysis revealed links between sulfur sources, nitrate reduction pathways, and microbial populations.