期刊
ENVIRONMENT INTERNATIONAL
卷 166, 期 -, 页码 -出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.envint.2022.107349
关键词
Handling Editor; Adrian Covaci; Nanoplastics; Proteomics; Metabolomics; mTORC1; Toxicity
资金
- National Natural Science Foundation of China [21974094, 42177412, 41722304]
- Open Project Program of the State Key Laboratory of Proteomics and Science [SKLP-O202004]
- Tianjin University Innovation Fund [2022XSU- 0008]
This study investigated the internalization, release, and cytotoxicity of polystyrene nanoplastics (PSNPs) in human cells and explored their potential effects on cellular metabolism using proteomic and metabolomic approaches. The findings revealed that PSNPs can affect cellular metabolism and inhibit the mTORC1 signaling pathway.
Nanoplastics (NPLs) are widespread in our environment. However, their impacts on human health and precise toxicity mechanisms remain poorly understood. Here we studied the internalization, release, and cytotoxicity of polystyrene nanoplastics (PSNPs) using the renal tubular epithelial cell line HKC and human derived liver cell line HL-7702. We also employed an integrated proteomic and metabolomic approach to investigate the potential biological effects of PSNPs on HKC cells. The abundances of 4770 proteins and 100 metabolites were quantified, with 785 proteins and 17 metabolites detected with altered levels in response to PSNPs. Most of the differential proteins and metabolites were enriched in a variety of metabolic pathways, for example, glycolysis, citrate cycle, oxidative phosphorylation, and amino acid metabolism, suggesting the potential effects of NPLs on global cellular metabolism shift in human cells. The altered energy metabolism induced by PSNPs was further confirmed by a Seahorse analysis. Moreover, lysosomal distribution study and western blotting showed that mTORC1 signaling, a central regulator of cellular metabolism, was inhibited upon nanoplastic exposure, likely serving as the link between lysosome dysfunction and metabolic defects. Taken together, our findings systematically mapped the key molecular changes induced by PSNPs in human cells and provide comprehensive biological insights for the risk estimation of NPLs contamination.
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