期刊
EMBO JOURNAL
卷 41, 期 15, 页码 -出版社
WILEY
DOI: 10.15252/embj.2021110472
关键词
intercellular bridge; microtubule; mitotic spindle; primary cilium; spastin
资金
- Ministry of Science and Technology (MOST), Taiwan [110-2636-B-007-011, 111-2636-B-007-009, A1101-0607]
- Ministry of Education (MOE), Taiwan [D110-H4001]
- National Tsing Hua University [111Q2713E1]
Microtubules, a crucial cellular structure, have been studied using chemo and optogenetics methods to disassemble specific types of microtubules. The results provide insights into their roles in cellular trafficking, organelle reorganization, and cell stiffness.
Microtubules tightly regulate various cellular activities. Our understanding of microtubules is largely based on experiments using microtubule-targeting agents, which, however, are insufficient to dissect the dynamic mechanisms of specific microtubule populations, due to their slow effects on the entire pool of microtubules. To overcome this technological limitation, we have used chemo and optogenetics to disassemble specific microtubule subtypes, including tyrosinated microtubules, primary cilia, mitotic spindles, and intercellular bridges, by rapidly recruiting engineered microtubule-cleaving enzymes onto target microtubules in a reversible manner. Using this approach, we show that acute microtubule disassembly swiftly halts vesicular trafficking and lysosomal dynamics. It also immediately triggers Golgi and ER reorganization and slows the fusion/fission of mitochondria without affecting mitochondrial membrane potential. In addition, cell rigidity is increased after microtubule disruption owing to increased contractile stress fibers. Microtubule disruption furthermore prevents cell division, but does not cause cell death during interphase. Overall, the reported tools facilitate detailed analysis of how microtubules precisely regulate cellular architecture and functions.
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