Determination of Creatinine in Urine by Voltammetry and Glassy Carbon Electrode Modified with Functionalized Multi-walled Carbon Nanotubes and Copper

A square-wave voltammetric method was developed for the determination of creatinine using glassy carbon electrode functionalized multi-walled carbon nanotubes and doped with copper. Since the creatine-creatinine balance is dependent on pH, the linear responses at either pH 4.0 or 7.0 were evaluated allowing versatility in application, covering analyte concentration from limit of quantification (0.3 mu mol L-1) up to 1.200 mu mol L-1 (pH 4) or 660 mu mol L-1 (pH 7). Electrode is simple to prepare and robust for routine analysis and it was used for the determination of creatinine in urine after simple thin-layer separation procedure to avoid interference from creatine.

Automated summary

A square-wave voltammetric method was developed for the determination of creatinine using glassy carbon electrode functionalized multi-walled carbon nanotubes and doped with copper. The linear responses at either pH 4.0 or 7.0 were evaluated allowing versatility in application. The electrode is simple to prepare and robust for routine analysis and it was used for the determination of creatinine in urine after simple thin-layer separation procedure to avoid interference from creatine.