期刊
ADVANCED FUNCTIONAL MATERIALS
卷 25, 期 21, 页码 3183-3192出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adfm.201500624
关键词
biopolymers; biosensing; graphene quantum dots; hybrid materials; ionic self-assembly
类别
资金
- EPSRC [EP/K020641/1, EP/K502352/1]
- ERC
- Engineering and Physical Sciences Research Council [EP/K502352/1, EP/K020641/1] Funding Source: researchfish
- EPSRC [EP/K020641/1, EP/K502352/1] Funding Source: UKRI
Hybrid self-assembly has become a reliable approach to synthesize soft materials with multiple levels of structural complexity and synergistic functionality. In this work, photoluminescent graphene quantum dots (GQDs, 2-5 nm) are used for the first time as molecule-like building blocks to construct self-assembled hybrid materials for label-free biosensors. Ionic self-assembly of disc-shaped GQDs and charged biopolymers is found to generate a series of hierarchical structures that exhibit aggregation-induced fluorescence quenching of the GQDs and change the protein/polypeptide secondary structure. The integration of GQDs and biopolymers via self-assembly offers a flexible toolkit for the design of label-free biosensors in which the GQDs serve as a fluorescent probe and the biopolymers provide biological function. The versatility of this approach is demonstrated in the detection of glycosaminoglycans (GAGs), pH, and proteases using three strategies: 1) competitive binding of GAGs to biopolymers, 2) pH-responsive structural changes of polypeptides, and 3) enzymatic hydrolysis of the protein backbone, respectively. It is anticipated that the integrative self-assembly of biomolecules and GQDs will open up new avenues for the design of multifunctional biomaterials with combined optoelectronic properties and biological applications.
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