期刊
DYES AND PIGMENTS
卷 204, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2022.110427
关键词
Photostability; Fluorescent probe; Cell organelle imaging; Multi-color imaging; Super-resolution imaging
资金
- Korea Health Industry Development Institute [HW20C2104]
- National Research Founda-tion of Korea [2009-0081571, RIAM0417-20150013, 2021R1A2C2005418]
- KIST intramural program
- National Research Foundation of Korea [2021R1A2C2005418] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Novel organelle-targeting fluorescent probes derived from NTD exhibit high photostability, fluorescence tunability, and organelle specificity with minimal cytotoxicity. These probes demonstrate intracellular differentiation of lysosomes and mitochondria in multi-color confocal imaging and have been successfully applied to super-resolution cell imaging techniques such as STED and SIM.
High-performance microscopy with organelle-targeting fluorescent probes has been an indispensable bioimaging tool for cell biology. Confocal or super-resolution imaging often requires highly magnified and/or longitudinal scanning conditions, necessitating the advent of photostable fluorophores. Herein, we report novel organelle targeting fluorescent probes derived from a highly photostable fluorophoric skeleton, i.e., 1,5-naphthyridine2,6-dione (NTD). With organelle-specific derivatization and pi-conjugation extention, we developed NTD-based molecular probes with blue-to-red fluorescence tunability and intracellular organelle selectivity for staining, as well as minimal cytotoxicity, which allowed us to demonstrate intracellular differentiation of lysosomes and mitochondria by multi-color confocal imaging. Under the condition of longitudinal microscopic examination, these new probes showed excellent photostability of fluorescent signals as opposed to the rapid photobleaching of commercial trackers. By virtue of these beneficial properties, the NTD-based probes were successfully applied to super-resolution cell imaging by enduring the high-power illumination conditions of sub-diffraction microscopic techniques including stimulated emission depletion (STED) and structured illumination microscopy (SIM).
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