期刊
DYES AND PIGMENTS
卷 203, 期 -, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.dyepig.2022.110305
关键词
Fluorescent probe; Benzothiazole; Cysteine; Cell imaging
资金
- National Natural Science Foundation of China [21878023, U1608222]
- Program for Distinguished Professor of Liaoning Province
- first-class discipline project of Liaoning province
- [LNSPXKBD2020307]
In this study, a series of fluorescent dyes based on 2-(2'-hydroxyphenyl)benzothiazole derivatives were designed and synthesized, which exhibited long-wavelength emission and a larger Stokes shift due to their structural characteristics of AIE + ESIPT. A fluorescent probe, HBTN-Y, was prepared based on the fluorescent dye HBTN and showed high selectivity towards cysteine, with OFF-ON fluorescence response, a large Stokes shift, and long-wavelength emission in a specific solution. The probe demonstrated good anti-interference ability, low detection limit, and applicability for live cell imaging.
A series of fluorescent dyes based on 2-(2'-hydroxyphenyl)benzothiazole derivatives were designed and synthesized. Because of their structural characteristics of AIE + ESIPT , they have long-wavelength emission and a larger Stokes shift. The fluorescent probe HBTN-Y was prepared based on the fluorescent dye HBTN. Probe HBTN-Y can recognize cysteine (Cys) with a high selectivity, OFF-ON fluorescence response, a large Stokes shift (263 nm) and long-wavelength emission (637 nm) in MeCN/H2O (1:1, v/v, PBS 20 mM, pH = 8.4) solution. The probe exhibits good anti-interference ability, a low detection limit of 6.51 x 10(-6) M, and is suitable for application at near neutral and weak alkaline pH (7-9) conditions. Moreover, HBTN-Y is applicable to image Cys in living MCF-7 cells.
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