4.6 Article

TLR5 recognizes Aeromonas hydrophila flagellin and interacts with MyD88 in Nile tilapia

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出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.dci.2022.104409

关键词

Nile tilapia ( Oreochromis niloticus ); TLR5; Immune response; NF-KB activation; Coimmunoprecipitation; Flagellin

资金

  1. National Key R&D Programme of China [2018YFD0900302]
  2. China Agriculture Research System of MOF [CARS-46]
  3. Guangdong Basic and Applied Basic Research Foundation [2021A1515010852]
  4. Guangdong Provincial Key RD Program [2021B0202020001]

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TLR5 gene was identified in Nile tilapia and found to play a crucial role in bacterial flagellin recognition and immune response.
Toll-like receptor 5 (TLR5) is responsible for bacterial flagellin recognition in vertebrates. In the present study, TLR5M was identified in the Nile tilapia Oreochromis niloticus (OnTLR5), containing a conserved LRR domain, a transmembrane region and a C-terminal TIR domain, similar to that of other fishes and mammals. OnTLR5 was broadly expressed in all the tissues examined, presenting the highest expression levels in the blood and the lowest in the kidney. OnTLR5 was detected from 2 d postfertilization (dpf) to 8 dpf during embryonic development. Moreover, expression levels of OnTLR5 were clearly altered in all five tissues examined in response to Streptococcus agalactiae infection in vivo. Overexpression of OnTLR5 in HEK293T cells revealed that OnTLR5 was distributed in the cytoplasm and significantly increased NF-KB activation. In response to cotransfection with OnMyd88, OnTLR5 significantly upregulated OnMyd88-induced NF-KB activation. Pulldown assays showed that OnTLR5 interacts with OnMyd88 and revealed an interaction between TLR5 and Aeromonas hydrophila flagellin. Taken together, these findings suggest that OnTLR5 plays important roles in TLR/IL-1R signalling pathways and the immune response to pathogen invasion.

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