An effect of choline lactate based low transition temperature mixtures on the lipase catalytic properties

A new series of low transition temperature mixures (LTTM) based on choline lactate quaternary ammonium salt and various hydrogen bond donors was prepared and characterized towards their physicochemical properties and usability as an enzymatic reaction mixture for lipase-catalyzed transesterification reactions. Studies of low transition temperature mixtures have shown a long-term stabilizing effect for lipase as well as a positive influence on lipase thermal stability. In the case of Ch[Lac]:Gly: EthGly increasing the stability of lipase by 8 C (up to 55.2 C) compared to the control sample. Conducted transesterification reactions were characterized by high yields - up to 98% - and high purity of the obtained products.

Automated summary

In this study, a new series of low transition temperature mixtures (LTTM) were prepared and evaluated for their physicochemical properties and usability in enzymatic transesterification reactions. The addition of various hydrogen bond donors increased the stability and thermal stability of lipase. The transesterification reactions conducted using these mixtures resulted in high yields and high purity of the products.