期刊
CNS NEUROSCIENCE & THERAPEUTICS
卷 28, 期 9, 页码 1315-1325出版社
WILEY
DOI: 10.1111/cns.13872
关键词
cell transplantation; glia; human pluripotent stem cells; leukodystrophy; Vanishing white matter
资金
- NWO Spinoza grant
- ZonMw VIDI research grant [91712343]
- ZonMw TAS IDB project [116005006]
- European Leukodystrophy Foundation [2014-012 l1]
- E-Rare Joint Call project [9003037601]
The transplantation of human glial cell populations has therapeutic effects for VWM. However, the microenvironment in the VWM patient brain should be considered as an important moderator of cell replacement therapy before further translation to the clinic.
Introduction Vanishing white matter (VWM) is a leukodystrophy that leads to neurological dysfunction and early death. Astrocytes are indicated as therapeutic target, because of their central role in VWM pathology. Previous cell replacement therapy using primary mouse glial precursors phenotypically improved VWM mice. Aims The aim of this study was to determine the translational potential of human stem cell-derived glial cell replacement therapy for VWM. We generated various glial cell types from human pluripotent stem cells in order to identify a human cell population that successfully ameliorates disease hallmarks of a VWM mouse model. The effects of cell grafts on motor skills and VWM brain pathology were assessed. Results Transplantation of human glial precursor populations improved the VWM phenotype. The intrinsic properties of these cells were partially reflected by cell fate post-transplantation, but were also affected by the host microenvironment. Strikingly, the spread of transplanted cells into the white matter versus the gray matter was different when grafted into the VWM brain as compared to a healthy brain. Conclusions Transplantation of human glial cell populations can have therapeutic effects for VWM. For further translation to the clinic, the microenvironment in the VWM patient brain should be considered as an important moderator of cell replacement therapy.
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