期刊
CHINESE CHEMICAL LETTERS
卷 33, 期 7, 页码 3484-3487出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2022.03.098
关键词
LA-sp-ICP-MS; Silver nanoparticle; Bioimaging; Degradation
资金
- National Natural Science Foundation of China [11975251, 11875268]
This study demonstrates the use of laser ablation-single particle inductively coupled plasma mass spectrometry (LA-sp-ICP-MS) for high-resolution, sensitive, and accurate imaging of the in situ distribution of particulate Ag and released ionic Ag in the sub-organs of mice after intravenous injection of Ag nanoparticles. The imaging results provide visual evidence of the accumulation and distribution of particulate Ag in the spleen and liver parenchyma, as well as the release and distribution of ionic Ag in the organs. The developed LA-sp-ICP-MS method is a powerful tool for studying the in vivo degradation behavior of metallic nanoparticles.
The in vivo degradation behavior of metallic nanoparticles (NPs) is very important for their biomedical applications and safety evaluation. Here, a method of laser ablation-single particle inductively coupled plasma mass spectrometry (LA-sp-ICP-MS) is shown to have high spatial resolution, sensitivity and accuracy for simultaneous imaging the in situ distribution of particulate Ag (P-Ag) and released ionic Ag (Ion-Ag) in the sub-organs of spleen, liver and kidney after intravenous injection of Ag nanoparticles (50 nm, AgNPs) to mice. Under the optimized parameters of 0.4 J/cm(2) laser fluence on a 30 pm spot with dwell time at 100 mu s, the signals of P-Ag and Ion-Ag in the organic tissues can be easily distinguished from the mass spectra. The method of iterative threshold algorithm has been used to distract the signals of P-Ag and Ion-Ag and separate each other. The resulting images for the first time provide visualized evidence that a considerable amount of P-Ag accumulated in the splenic marginal zone, but widely distributed in the liver parenchyma at 24 h after injection of AgNPs, and in the meantime, obvious amounts of ionic Ag released and distributed in the organs. In addition, the imaging results indicate that the AgNP excretion in the kidney is mainly in ionic forms. The investigation here demonstrates that the developed LA-sp-ICP-MS method with high spatial resolution, sensitivity and visualization capability can become a powerful tool in the clinical context of metallic NPs. (C) 2022 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
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