期刊
CHEMMEDCHEM
卷 17, 期 18, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cmdc.202200300
关键词
Alzheimer's disease; acetylcholinesterase; butyrylcholinesterase; coumarin-donepezil hybrid; BACE-1
资金
- CSIR Phytopharmaceutical Mission [HCP-010]
Plants, particularly Nardostachys jatamansi, have been explored for their potential in drug discovery for treating neurodegenerative diseases. This study focused on the phytochemical investigation and semisynthetic modifications of N. jatamansi rhizomes to discover inhibitors for cholinesterase and BACE-1. Several compounds, including 8-acetyl-7-hydroxycoumarin and coumarin-donepezil hybrid, showed promising inhibitory effects on the target enzymes.
Plants have immensely contributed to the drug discovery for neurodegenerative diseases. Herein, we undertook the phytochemical investigation of Nardostachys jatamansi (D.Don) DC. rhizomes followed by semisynthetic modifications to discover cholinesterase (ChE) and beta-site amyloid precursor protein cleaving enzyme 1 (BACE-1) inhibitors. The 8-acetyl-7-hydroxycoumarin isolated from the bioactive extract moderately inhibits acetylcholinesterase (AChE) and BACE-1 with IC50 values of 22.1 and 17.7 mu M, respectively. The semisynthetic trifluoromethyl substituted coumarin chalcone display a 5-fold improvement in BACE-1 inhibition (IC50 3.3 mu M). Another semisynthetic derivative, a coumarin-donepezil hybrid, exhibits dual inhibition of both ChEs with IC50 values of 1.22 and 3.09 mu M, respectively. Molecular modeling and enzyme kinetics revealed that the coumarin-donepezil hybrid is a non-competitive inhibitor of AChE. It crosses the blood-brain barrier and also inhibits A beta self-aggregation. The results presented herein warrant a detailed investigation of the coumarin-donepezil hybrid in preclinical models of Alzheimer's disease.
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