Protein enrichment by foam Fractionation: Experiment and modeling

This work investigates the enrichment of bovine serum albumin (BSA) protein through foam fractionation. Here, we performed experiments using BSA and measured the recovery and grade of the extract. Additionally, an unsteady-state simulation of the protein foam fractionation process was carried out by numerically solving the liquid drainage equation in the foam. Thereby, the extracted liquid volume and protein concentration were calculated. Required quantities such as foam stability, interface coverage or bubble size distribution were measured in corresponding experiments and were fed into the model. The experiments showed that the foam coalescence accelerates the liquid drainage leading to dryer extract and higher protein enrichment. The modeling also reproduced the liquid recovery and extract concentration of the foam fractionation tests within a reasonable error range. The modeling solely relies on experimental inputs and does not require any tuning parameters. It can be further used for optimization or up-scaling of protein foam fractionation. (C) 2022 Published by Elsevier Ltd.

Automated summary

This study investigates the enrichment of bovine serum albumin protein through foam fractionation. Experiments and numerical simulations were conducted to analyze the recovery and grade of the extract. The results showed that foam coalescence accelerates liquid drainage, leading to a drier extract and higher protein enrichment. The simulation model successfully reproduced the experimental results within a reasonable error range, providing a useful tool for optimization and up-scaling of protein foam fractionation.