4.4 Article

A High-Throughput Continuous Spectroscopic Assay to Measure the Activity of Natural Product Methyltransferases

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Chemistry, Multidisciplinary

Multienzyme One-Pot Cascades Incorporating Methyltransferases for the Strategic Diversification of Tetrahydroisoquinoline Alkaloids

Fabiana Subrizi et al.

Summary: This study demonstrates the use of methyltransferases in multi-enzyme cascades in vitro, including the generation of SAM in situ, to achieve regioselective diversification of THIQs. Regioselectivities of the methyltransferases depend on the group at C-1 and the presence of fluorine in different THIQs. Additionally, catechol methyltransferases used in the study show interesting dual activity.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Review Biotechnology & Applied Microbiology

Biotechnological applications of S-adenosyl-methionine-dependent methyltransferases for natural products biosynthesis and diversification

Congqiang Zhang et al.

Summary: Methylation is a crucial transformation in the biosynthesis of natural products, and recent advancements in identifying novel methyltransferases, enzyme engineering strategies, and co-factor regeneration methods have contributed to enhancing bioactivity and yield of these products. Promiscuous methyltransferases have shown versatility in accepting multiple structurally similar substrates, allowing for the design of pathways to produce molecules not found in nature, expanding the range of biosynthesized functional molecules.

BIORESOURCES AND BIOPROCESSING (2021)

Article Biochemistry & Molecular Biology

A bicyclic S-adenosylmethionine regeneration system applicable with different nucleosides or nucleotides as cofactor building blocks

Desiree Popadic et al.

Summary: SAM is a common cofactor involved in many biochemical reactions, particularly in metabolism, epigenetics, and cancer development. An optimized biomimetic system has been developed for the regeneration of SAM and its analogues, utilizing effective nucleoside triphosphate formation and an additional l-methionine regeneration cycle.

RSC CHEMICAL BIOLOGY (2021)

Review Biotechnology & Applied Microbiology

Protein Engineering for Improving and Diversifying Natural Product Biosynthesis

Chenyi Li et al.

TRENDS IN BIOTECHNOLOGY (2020)

Article Chemistry, Physical

S-adenosylhomocysteine as a methyl transfer catalyst in biocatalytic methylation reactions

Cangsong Liao et al.

NATURE CATALYSIS (2019)

Article Chemistry, Multidisciplinary

Catalytic Alkylation Using a Cyclic S-Adenosylmethionine Regeneration System

Silja Mordhorst et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Review Biotechnology & Applied Microbiology

Natural product discovery: past, present, and future

Leonard Katz et al.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2016)

Article Biochemistry & Molecular Biology

Regiocomplementary O-Methylation of Catechols by Using Three-Enzyme Cascades

Jutta Siegrist et al.

CHEMBIOCHEM (2015)

Article Genetics & Heredity

A continuous kinetic assay for protein and DNA methyltransferase enzymatic activities

Shai Duchin et al.

EPIGENETICS & CHROMATIN (2015)

Review Chemistry, Multidisciplinary

Profound Methyl Effects in Drug Discovery and a Call for New C-H Methylation Reactions

Heike Schoenherr et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2013)

Article Chemistry, Multidisciplinary

Structure-Guided Discovery of New Deaminase Enzymes

Daniel S. Hitchcock et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2013)

Review Biochemistry & Molecular Biology

Architectures, mechanisms and molecular evolution of natural product methyltransferases

David K. Liscombe et al.

NATURAL PRODUCT REPORTS (2012)

Article Biochemical Research Methods

In silico analysis of methyltransferase domains involved in biosynthesis of secondary metabolites

Mohd Zeeshan Ansari et al.

BMC BIOINFORMATICS (2008)

Article Biotechnology & Applied Microbiology

Characterization of SafC, a catechol 4-O-methyltransferase involved in saframycin biosynthesis

James T. Nelson et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2007)

Review Biochemistry & Molecular Biology

The value of natural products to future pharmaceutical discovery

Dwight D. Baker et al.

NATURAL PRODUCT REPORTS (2007)

Article Biochemical Research Methods

An enzyme-coupled continuous spectrophotometric assay for S-adenosylmethionine-dependent methyltransferases

KM Dorgan et al.

ANALYTICAL BIOCHEMISTRY (2006)

Article Biochemistry & Molecular Biology

A general fluorescence-based coupled assay for S-adenosylmethionine-dependent methyltransferases

CH Wang et al.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (2005)

Article Biochemical Research Methods

An enzyme-coupled colorimetric assay for S-adenosylmethionine-dependent methyltransferases

CL Hendricks et al.

ANALYTICAL BIOCHEMISTRY (2004)