A High-Throughput Continuous Spectroscopic Assay to Measure the Activity of Natural Product Methyltransferases

E.coli BL21(DE3) cells carrying the pMHT1 TmMtaD expression vector are a kind gift from Prof. Amir Aharoni and Prof. Dan Levi, Ben-Gurion University of the Negev, Israel. The plasmid pET28 carrying the MjDadD gene was a kind gift from Prof. Jennifer Andexer, University of Freiburg, Germany. The authors acknowledge the Federal Ministry of Education and Research (BMBF, Germany) (161B0626 A) for M. R. and the Israel Ministry of Health (ERA-NET 5317) for S. B. for funding in frame of the 17-ERACoBioTech as part of the project BioDiMet: Methyl Transferases for the Functional Diversification of Bioactives.

Automated summary

This article discusses the sources of E. coli BL21(DE3) cells carrying the pMHT1 TmMtaD expression vector and the plasmid pET28 carrying the MjDadD gene, as well as the acknowledgment of funding from two departments.