期刊
CHEMBIOCHEM
卷 23, 期 16, 页码 -出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cbic.202200265
关键词
fucoidan; hepatocellular carcinoma; iron oxide nanoparticles; natural polysaccharides; proteome
资金
- Region Ile-de-France
This study compared the bioactivity of free fucoidan and fucoidan-loaded nanoparticles in human hepatoma cells. The results showed that nanoparticles reduced cell invasion and migration, while free fucoidan increased these events at low concentrations. Moreover, nanoparticles decreased the production of reactive oxygen species and the activity of matrix metalloproteinase-9 compared to free fucoidan. Proteomic analysis revealed that fucoidan regulated proteins associated with protein synthesis, N-glycan processing, and cellular stress.
Fucoidan is a natural sulfated polysaccharide with a large range of biological activities including anticancer and anti-oxidation activities. Hepatocellular carcinoma is the fourth most common aggressive cancer type. The aim of this study was to investigate the bioactivity of free fucoidan versus its vectorization using nanoparticles (NPs) in human hepatoma cells, Huh-7. Iron oxide NPs were functionalized with fucoidan by a one-step surface complexation. NP cellular uptake was quantified by magnetic measurement at various extracellular iron concentrations. Cell invasion and migration were reduced with NPs while free fucoidan increases these events at low fucoidan concentration (<= 0.5 mu M). Concomitantly, a high decrease of reactive oxygen species production related with a decrease of the matrix metalloproteinase-9 activity and an increase of its expression was observed with NPs compared to free fucoidan. A proteomic analysis evidenced that some fucoidan regulated proteins appeared, which were related to protein synthesis, N-glycan processing, and cellular stress. To our knowledge, this is the first study which reveals such activity induced by fucoidan. These results pave the way for USPIO-fucoidan-NPs as potential theranostic nanotools for hepatocellular carcinoma treatment.
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