期刊
CELLULAR SIGNALLING
卷 95, 期 -, 页码 -出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cellsig.2022.110341
关键词
COPS3 AS lncRNA; miR-762; MyoD; Myogenesis; Myotube phenotype
类别
资金
- National Key Research and Devel-opment Program of China [2021YFF1000602]
- China Agriculture Research System of MOFand MARA [CARS-35]
- Key Research and Development Program of Shaanxi Province [2022ZDLNY01-04]
- Natural Science Foundation of Shaanxi Province [2022JQ-170]
COPS3 AS lncRNA is identified as a key regulator in myogenesis, functioning through its interaction with miR-762 to promote myogenic differentiation and Fast-MyHC expression.
Long non-coding RNAs (lncRNAs) play essential roles in myogenesis. Here, we identified a novel long non-coding RNA, named COPS3 AS lncRNA (COP9 signalosome complex subunit 3 antisense lncRNA), which was transcribed from the mouse COPS3 gene antisense strand and highly expressed in glycolytic muscle fibers. Functionally, COPS3 AS lncRNA knockdown inhibited myogenic differentiation in myoblasts, whereas its overexpression promoted the process. Moreover, COPS3 AS lncRNA maintained the fast-twitch myotubes phenotype. Mecha-nistically, although COPS3 AS lncRNA did not form AS lncRNA/mRNA dimer with COPS3 mRNA, it as a competing endogenous RNA (ceRNA) to sponge miR-762, promoted myogenic differentiation and Fast-MyHC expression by modulating miR-762 target gene myogenic differentiation 1 (MyoD1). Taken together, COPS3 AS lncRNA is a key candidate regulator of myogenesis and fast-MyHC myotubes specification by miR-762/MyoD signalling axis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据