4.7 Article

Full-length transcriptome analysis of maize root tips reveals the molecular mechanism of cold stress during the seedling stage

期刊

BMC PLANT BIOLOGY
卷 22, 期 1, 页码 -

出版社

BMC
DOI: 10.1186/s12870-022-03787-3

关键词

Cold stress; Full-length transcriptome; Seedling stage; Maize; ROS; Amine

资金

  1. special project of Guangdong Academy of Sciences, China [2020GDASYL-20200103073]
  2. Basic and Applied Basic Research Fund of Guangdong Province [2021A1515110745]
  3. National Natural Science Foundation of China [32072027]
  4. China Agricultural Research System [CARS201707]
  5. Laboratory of Lingnan Modern Agriculture Project [NZ2021014]

向作者/读者索取更多资源

Our study on cold tolerant maize inbred lines identified numerous up-regulated transcripts under cold stress, including those involved in reactive oxygen species metabolism and heat response. Eight genes exhibited specific differential alternative splicing in the cold tolerant line, mainly related to amine metabolism. Additionally, a total of 1,111 lncRNAs were identified, with 62 of them up-regulated under cold stress in both inbred lines, enriched in protein phosphorylation.
Background As maize originated in tropical or subtropical zones, most maize germplasm is extremely sensitive to low temperatures during the seedling stage. Clarifying the molecular mechanism of cold acclimation would facilitate the breeding of cold tolerant maize varieties, which is one of the major sustainability factors for crop production. To meet this goal, we investigated two maize inbred lines with contrasting levels of cold tolerance at the seedling stage (IL85, a cold tolerant line; B73, a cold sensitive line), and performed full-length transcriptome sequencing on the root tips of seedlings before and after 24 h of cold treatment. Results We identified 152,263 transcripts, including 20,993 novel transcripts, and determined per-transcript expression levels. A total of 1,475 transcripts were specifically up-regulated in the cold tolerant line IL85 under cold stress. GO enrichment analysis revealed that 25 transcripts were involved in reactive oxygen species (ROS) metabolic processes and 15 transcripts were related to the response to heat. Eight genes showed specific differential alternative splicing (DAS) in IL85 under cold stress, and were mainly involved in amine metabolism. A total of 1,111 lncRNAs were further identified, 62 of which were up-regulated in IL85 or B73 under cold stress, and their corresponding target genes were enriched in protein phosphorylation. Conclusions These results provide new insights into the molecular mechanism of cold acclimation during the seedling stage in maize, and will facilitate the development of cultivars with improved cold stress tolerance.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据