A gossypol derivative effectively protects against Zika and dengue virus infection without toxicity

Background Zika virus (ZIKV) and dengue virus (DENV) cause microcephaly and dengue hemorrhagic fever, respectively, leading to severe problems. No effective antiviral agents are approved against infections of these flaviviruses, calling for the need to develop potent therapeutics. We previously identified gossypol as an effective inhibitor against ZIKV and DENV infections, but this compound is toxic and not suitable for in vivo treatment. Results In this study, we showed that gossypol derivative ST087010 exhibited potent and broad-spectrum in vitro inhibitory activity against infections of at least ten ZIKV strains isolated from different hosts, time periods, and countries, as well as DENV-1-4 serotypes, and significantly reduced cytotoxicity compared to gossypol. It presented broad-spectrum in vivo protective efficacy, protecting ZIKV-infected Ifnar1(-/-) mice from lethal challenge, with increased survival and reduced weight loss. Ifnar1(-/-) mice treated with this gossypol derivative decreased viral titers in various tissues, including the brain and testis, after infection with ZIKV at different human isolates. Moreover, ST087010 potently blocked ZIKV vertical transmission in pregnant Ifnar1(-/-) mice, preventing ZIKV-caused fetal death, and it was safe for pregnant mice and their pups. It also protected DENV-2-challenged Ifnar1(-/-) mice against viral replication by reducing the viral titers in the brain, kidney, heart, and sera. Conclusions Overall, our data indicate the potential for further development of this gossypol derivative as an effective and safe broad-spectrum therapeutic agent to treat ZIKV and DENV diseases.

Automated summary

The gossypol derivative ST087010 exhibits potent and broad-spectrum inhibitory activity against various strains of ZIKV and serotypes of DENV, with reduced cytotoxicity compared to gossypol. It provides broad-spectrum protection in vivo, decreasing viral titers in different tissues and preventing vertical transmission of ZIKV in pregnant mice. Moreover, it reduces viral titers in various organs and protects against viral replication in DENV-challenged mice. Overall, this gossypol derivative shows potential as an effective and safe broad-spectrum therapeutic agent for ZIKV and DENV diseases.