期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 119, 期 11, 页码 3297-3310出版社
WILEY
DOI: 10.1002/bit.28202
关键词
3D printed; bone marrow mesenchymal stem cells; computational fluid dynamics; osteogenic differentiation; silicate-substituted calcium phosphate; tissue engineering
资金
- Natural Science Foundation of Heilongjiang Province of China
- National Natural Science Foundation of China
The study used digital light processing (DLP) printing technology to fabricate a novel porous 3D printed Si-CaP scaffold, which exhibited a uniform spatial structure, appropriate mechanical properties, and effective interior permeability. Compared to conventional Si-CaP ceramics, the 3D printed Si-CaP scaffold showed sustained release of ions and enhanced osteogenic effects, improving the adhesion, proliferation, and osteogenic differentiation of bone marrow mesenchymal stem cells.
Silicate-substituted calcium phosphate (Si-CaP) ceramics, alternative materials for autogenous bone grafting, exhibit excellent osteoinductivity, osteoconductivity, biocompatibility, and biodegradability; thus, they have been widely used for treating bone defects. However, the limited control over the spatial structure and weak mechanical properties of conventional Si-CaP ceramics hinder their wide application. Here, we used digital light processing (DLP) printing technology to fabricate a novel porous 3D printed Si-CaP scaffold to enhance the scaffold properties. Scanning electron microscopy, compression tests, and computational fluid dynamics simulations of the 3D printed Si-CaP scaffolds revealed a uniform spatial structure, appropriate mechanical properties, and effective interior permeability. Furthermore, compared to Si-CaP groups, 3D printed Si-CaP groups exhibited sustained release of silicon (Si), calcium (Ca), and phosphorus (P) ions. Furthermore, 3D printed Si-CaP groups had more comprehensive and persistent osteogenic effects due to increased osteogenic factor expression and calcium deposition. Our results show that the 3D printed Si-CaP scaffold successfully improved bone marrow mesenchymal stem cells (BMSCs) adhesion, proliferation, and osteogenic differentiation and possessed a distinct apatite mineralization ability. Overall, with the help of DLP printing technology, Si-CaP ceramic materials facilitate the fabrication of ideal bone tissue engineering scaffolds with essential elements, providing a promising approach for bone regeneration.
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