期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 119, 期 9, 页码 2529-2540出版社
WILEY
DOI: 10.1002/bit.28159
关键词
metabolic engineering; 2-pyrone synthase; oilcane juice; Rhodotorula toruloides; triacetic acid lactone
资金
- U.S. Department of Energy [DE-SC0018420]
This study demonstrates the metabolic engineering of Rhodotorula toruloides to achieve high production of the plant-sourced polyketide triacetic acid lactone (TAL). By introducing relevant genes and optimizing metabolic pathways, TAL production was significantly increased. The findings highlight the potential of Rhodotorula toruloides as a host for the production of TAL and other polyketides from low-cost carbon sources.
The plant-sourced polyketide triacetic acid lactone (TAL) has been recognized as a promising platform chemical for the biorefinery industry. However, its practical application was rather limited due to low natural abundance and inefficient cell factories for biosynthesis. Here, we report the metabolic engineering of oleaginous yeast Rhodotorula toruloides for TAL overproduction. We first introduced a 2-pyrone synthase gene from Gerbera hybrida (GhPS) into R. toruloides and investigated the effects of different carbon sources on TAL production. We then systematically employed a variety of metabolic engineering strategies to increase the flux of acetyl-CoA by enhancing its biosynthetic pathways and disrupting its competing pathways. We found that overexpression of ATP-citrate lyase (ACL1) improved TAL production by 45% compared to the GhPS overexpressing strain, and additional overexpression of acetyl-CoA carboxylase (ACC1) further increased TAL production by 29%. Finally, we characterized the resulting strain I12-ACL1-ACC1 using fed-batch bioreactor fermentation in glucose or oilcane juice medium with acetate supplementation and achieved a titer of 28 or 23 g/L TAL, respectively. This study demonstrates that R. toruloides is a promising host for the production of TAL and other acetyl-CoA-derived polyketides from low-cost carbon sources.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据