期刊
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
卷 36, 期 1, 页码 439-447出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/13102818.2022.2095303
关键词
Succinic acid; Actinobacillus succinogenes; xylose mother liquor; Plackett-Burman design; response surface methodology
资金
- Science and Technology Major Project of Guangxi [AA18242026, AB21196019, AB21220020]
- National Natural Science Foundation of Guangxi Province [2018GXNSFAA28113, 2019GXNSF AA185003]
- National Natural Science Foundation of China [32060020, 31660022, 32160017]
- Scientific Research Project for Introducing High-level Talents of Guangxi University for Nationalities [2018KJQD17]
- Innovation Project of Guangxi Graduate Education [gxun-chxps202078]
- Guangxi Graduate Education Innovation Plan Project [YCSW2021156]
This study optimized the conditions for succinic acid production using xylose mother liquor as the culture medium. Through a series of experiments and design methods, the optimal SA fermentation conditions were determined, resulting in high yields of SA.
In the present study, conditions for succinic acid (SA) production using xylose mother liquor (XML) as culture medium by Actinobacillus succinogenes GXAS137 were optimized. Firstly, single-factor experiments were performed to evaluate the basal culture medium for SA fermentation. Thereafter, the Plackett-Burman design was used to screen out three significant factors of XML, corn steep liquor powder (CSLP) and MgCO3 affecting the SA yields from the original nine factors. Subsequent use of steepest ascent experiment determined the center area of the three factors. Finally, the response surface methodology was used to further optimize the interactions between the three main factors and predict the maximum SA concentration through Box-Behnken design. The optimal conditions of SA fermentation were maximally documented in the XML (110 g/L), CSLP (18.86 g/L) and MgCO3 (69.12 g/L). The maximum production of SA was 58.06 +/- 0.57 g/L after 60 h with a yield of 0.72 +/- 0.06 g/g total sugar, approaching the predicted value (57.99 g/L). It was 1.63-fold of the SA production obtained with the basic medium (35.54 g/L). In addition, batch fermentations were carried out in a 1.3-L stirred bioreactor and SA reached 58.47 g/L. These results indicate that XML could be an alternative substrate for the economical production of SA by A. succinogenes GXAS137.
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