期刊
BIOSENSORS & BIOELECTRONICS
卷 207, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2022.114148
关键词
Gold nanoflowers; Red phosphorus; Recyclable immunoassay; Surface-enhanced Raman scattering
类别
资金
- Natural Science Foundation of Zhejiang Province [LY19F050002]
- Fundamental Research Funds for the Provincial Universities of Zhejiang [SJLY2020012]
- K. C. Wong Magna Fund in Ningbo University, China
This study proposes a recyclable immunoassay method utilizing the synergistic effects of electromagnetic and chemical interactions, based on surface-enhanced Raman scattering (SERS) technology, to detect tumor markers in clinical serum samples with high sensitivity. By growing sea-urchin-like gold nanoflowers on two-dimensional red phosphorus nanoplates, efficient enhancement of immunoassay, as well as stable photocatalytic and SERS activities, were achieved. With the combination of a non-metallic immunoprobe, the limit of detection for cancer antigen 19-9 was significantly reduced, demonstrating promising selectivity. These multifunctional red phosphorus/gold nanoflowers have great potential in early cancer detection and point-of-care diagnosis.
It is tremendously desirable for the timely and effective detection of cancer to facilitate the ultra-highly sensitive monitoring of tumor marker in clinical serum sample. In this study, an electromagnetic and chemical synergistically enabled recyclable immunoassay based on surface-enhanced Raman scattering (SERS) was proposed by realizing the anisotropic growth of sea-urchin-like gold nanoflowers (Au NFs) on two-dimensional red phosphorus (RP) nanoplates. Besides the achieved enhancement factor as high as 2.24 x 10(6), it was found that the photocatalytic and SERS activities were kept at a high level for the hybrid substrate of RP/Au NFs throughout 7 cycles of immunoassay. In combination with a non-metallic immunoprobe, the limit of detection was drove to 7.41 x 10(-5) IU.mL(-1) for cancer antigen 19-9. The comparative experiments of nonspecific monitoring verified the promising selectivity of this strategy. Considering the intriguing features of high sensitivity, recyclability, and specificity, the proposed multifunctional RP/Au NFs exhibited its superior role in the early detection of cancer and can be adapted for point-of-care diagnosis.
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