期刊
BIOSENSORS & BIOELECTRONICS
卷 210, 期 -, 页码 -出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2022.114211
关键词
Biomarkers; Neurotransmitter; Electrochemical sensors; Neurodegenerative diseases; Blood analysis
类别
资金
- Coordination for the Improvement of Higher Education Personnel (CAPES)
- National Council for Scientific and Technological Development (CNPq) [305133/2019-5, 06/2019-Silmar Silva Teixeira]
- Research Productivity Grant
- [431275/2018-1]
- [28/2018-Universal/Range B-Carla Eiras]
- [311802/2017-6]
- [12/2017-Carla Eiras]
A novel composite material was developed and applied for the detection of neurotransmitter dopamine. The composite exhibited high selectivity and sensitivity, allowing for direct detection in human serum without sample pretreatment. The proposed method showed promising potential for clinical diagnosis.
Composite materials have gained significant attention owing to the synergistic effects of their constituent materials, thereby facilitating their utilization in new applications or in improving the existing ones. In this study, a composite based on nickel phthalocyanine (NiTsPc), zinc oxide nanoparticles (ZnONPs), and carbon nanotubes (CNT) was developed and subsequently immobilized on a pyrolytic graphite electrode (PGE). The PGE/NiTsPcZnONPs-CNT was identified as a selective catalytic hybrid system for detection of neurotransmitter dopamine (DA). The electrochemical and morphological characterizations were conducted using atomic force microscopy (AFM). Chronoamperometry and differential pulse voltammetry (DPV) were used to detect DA and detection limits of 24 nM and 7.0 nM was found, respectively. In addition, the effects of some possible DA interferents, such as ascorbic acid, uric acid, and serotonin, on DA response were evaluated. Their presence did not show significant variations in the DA electrochemical response. The high specificity and sensitivity of PGE/NiTsPc-ZnONPsCNT for DA enabled its direct detection in human serum without sample pretreatment as well as in DA-enriched serum samples, whose recovery levels were close to 100%, thereby confirming the effectiveness of the proposed method. In general, PGE/NiTsPc-ZnONPs-CNT is a promising candidate for future applications in clinical diagnosis.
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